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KMID : 0380020040190040274
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Korean Journal of Biotechnology and Bioengineering
2004 Volume.19 No. 4 p.274 ~ p.282
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Pharmaceutical Characteristics of Korean Lumbricus rubellus Lumbrokinase
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Cho Il-Hwan
Lee Chul-Kyu
Lim Heon-Gil
Lee Hyung-Hoan
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Abstract
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Six lumbrokinase (LK) fractions from Lumbricus rubellus lysates were purified by a series of column chromatographies. The molecular weights of the six LK fractions appeared to range from 24.6 to 33.1 kDa. In the experimental model of rat venous thrombosis, the thrombus weight and PAI activity decreased significantly when the LK was administered orally. However, the activities of APTT, PT and plasmin showed a significant increase. The aggregation of rat platelets pretreated with various LK doses was inhibited by thrombin, and the MDA generation decreased. The rat thoracic aorta and mesentric arteries contracted with phenylephrine relaxed due to the treatment of the LK fractions. These results suggest that the fibrinolytic effects of LK were mediated not only by proteolytic activity, but also by the inhibition of platelet agregation and the relaxation of blood vessels. It is concluded that the LK may be useful as a hemolytic agent for treatment of fibrin clot.
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KEYWORD
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Lumbricus rubellus, lumbrokinase, fibrinolytic effects, fibrin clot, thrombin
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